Journal: Scientific Reports

Article Title: Delphinidin upregulates microRNA-let-7b expression through family with sequence similarity 222 member B

doi: 10.1038/s41598-025-15588-3

Figure Lengend Snippet: Identification of molecules that regulate the growth inhibitory effects of melanoma cells by delphinidin. ( a ) Schematic of genetic screening for molecules that mediate the inhibition of melanoma cell proliferation by delphinidin. ( b ) Fam222B protein levels in melanoma cell lines and NHEMs were assessed using Western blot analysis ( n = 1). ( c and d ) Fam222B mRNA expression and protein levels were evaluated in B16 cells treated with delphinidin for 48 h ( n = 3). Data are presented as mean ± SEM. Statistical analysis was conducted using one-way ANOVA followed by Dunnett’s multiple comparison test.

Article Snippet: Mouse melanoma B16 cells, human melanoma cell lines (A375, Hs294t, and MeWo), and normal human epidermal melanocytes (NHEMs) were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Inhibition, Western Blot, Expressing, Comparison